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The rest of the paper is organized as follows: In Section 2 we provide an overview of Star Trek film and television series franchise. In Section 3 we introduce the LTO version 0.1.1. It is a hierarchically organized controlled vocabulary of themes, partitioned into the following four domains: the human condition, society, the pursuit of knowledge and alternate reality. There are 1535 unique themes in total. Criteria and guidelines motivating our hierarchical arrangement are discussed. In Section 4 we explain the hypergeometric testing approach to theme enrichment analysis in full technical detail. In Section 5 we use the hypergeometric test to identify enriched themes in the two Star Trek case studies outlined above. These results we compare with those obtained using the standard TF-IDF approach to enrichment analysis. We conclude the paper in Section 6 with a summary of our main contributions, a discussion of some limitations of our methodology and go on to describe a handful of possible future directions. Most notably, in terms of limitations, we emphasize that we manually annotated Star Trek episodes with themes, and as a consequence the findings we report inevitably reflect our point of view, and are not fully replicable. The theme enrichment analysis procedure based on the hypergeometric test is implemented in the R package stoRy (version 0.1.1) (Sheridan and Onsjö 2017), released through CRAN (The Comprehensive R Archive Network 2019). The thematically annotated Star Trek episode dataset is included in the package. A related R Shiny web application is available for download at the Theme Ontology GitHub repository (Theme Ontology Project GitHub Repository 2019). 2b1af7f3a8